The purpose of this project is first, to quantify the amount of GTC present in waste from VL and EID testing platforms, and then identify and develop procedures for sustainable, environmentally-friendly and occupationally-safe methods to treat GTC containing HIV VL and EID testing waste in resource limited situations. The initial plan for this project is to complete the listing of VL platforms and estimate the GTC chemical composition of waste generated by these commonly used platforms for HIV VL testing. Next steps, would involve research, development and application of laboratory-scale mitigation methods, including GTC test method validation, GTC containing waste measurement, treatment optimization and validation, and data analysis. The combination of data analysis and experience gained from the laboratory-scale exercises will afford the opportunity to conduct a future in-country application of the methods developed. A report of our findings will be used to develop a GTC containing waste mitigation testing plan that will be conducted in various countries during the course of the project. Ultimately, guidance, SOPs, and job aids will be an output that will be distributed to all PEPFAR supported countries.